mitochondrial membrane potential detection kit jc 1 Search Results


hy k0601 100t  (MedChemExpress)
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MedChemExpress hy k0601 100t
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jc 1 probe  (Biotium)
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Biotium jc 1 probe
Jc 1 Probe, supplied by Biotium, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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membrane potential assay kit  (Beyotime)
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Beyotime membrane potential assay kit
Membrane Potential Assay Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mitochondrial membrane potential assay kit  (Beijing Solarbio Science)
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Beijing Solarbio Science mitochondrial membrane potential assay kit
Fig. 2. Silica exposure induces <t>mitochondrial</t> depolarization and disrupts energy homeostasis in RAW-ASC cells. RAW-ASC cells were primed with LPS for 6 h then treated with nano-silica, micro-silica, and ATP for 4 h, respectively. (A) Representative images of JC-1 staining showing the alteration of mitochondrial membrane potential between groups. Red, JC-1 aggregates; Green, JC-1 monomers. Scale bar = 50 µm. (B) Quantitative analysis and comparison of fluorescence intensity in A between groups (n = 6). (C) Measurement and comparison of intracellular ROS content between groups (n = 6). (D) Measurement and comparison of intracellular ATP content between groups (n = 4). ns, not significant, *P < 0.05, **P < 0.01.
Mitochondrial Membrane Potential Assay Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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jc-1 dye  (Beijing Solarbio Science)
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Beijing Solarbio Science jc-1 dye
Fig. 2. Silica exposure induces <t>mitochondrial</t> depolarization and disrupts energy homeostasis in RAW-ASC cells. RAW-ASC cells were primed with LPS for 6 h then treated with nano-silica, micro-silica, and ATP for 4 h, respectively. (A) Representative images of JC-1 staining showing the alteration of mitochondrial membrane potential between groups. Red, JC-1 aggregates; Green, JC-1 monomers. Scale bar = 50 µm. (B) Quantitative analysis and comparison of fluorescence intensity in A between groups (n = 6). (C) Measurement and comparison of intracellular ROS content between groups (n = 6). (D) Measurement and comparison of intracellular ATP content between groups (n = 4). ns, not significant, *P < 0.05, **P < 0.01.
Jc 1 Dye, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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jc-1 staining solution  (Cayman Chemical)
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Cayman Chemical jc-1 staining solution
Fig. 2. Silica exposure induces <t>mitochondrial</t> depolarization and disrupts energy homeostasis in RAW-ASC cells. RAW-ASC cells were primed with LPS for 6 h then treated with nano-silica, micro-silica, and ATP for 4 h, respectively. (A) Representative images of JC-1 staining showing the alteration of mitochondrial membrane potential between groups. Red, JC-1 aggregates; Green, JC-1 monomers. Scale bar = 50 µm. (B) Quantitative analysis and comparison of fluorescence intensity in A between groups (n = 6). (C) Measurement and comparison of intracellular ROS content between groups (n = 6). (D) Measurement and comparison of intracellular ATP content between groups (n = 4). ns, not significant, *P < 0.05, **P < 0.01.
Jc 1 Staining Solution, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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jc-1 mitochondrial membrane potential assay kit  (Abnova)
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Abnova jc-1 mitochondrial membrane potential assay kit
Fig. 2. Silica exposure induces <t>mitochondrial</t> depolarization and disrupts energy homeostasis in RAW-ASC cells. RAW-ASC cells were primed with LPS for 6 h then treated with nano-silica, micro-silica, and ATP for 4 h, respectively. (A) Representative images of JC-1 staining showing the alteration of mitochondrial membrane potential between groups. Red, JC-1 aggregates; Green, JC-1 monomers. Scale bar = 50 µm. (B) Quantitative analysis and comparison of fluorescence intensity in A between groups (n = 6). (C) Measurement and comparison of intracellular ROS content between groups (n = 6). (D) Measurement and comparison of intracellular ATP content between groups (n = 4). ns, not significant, *P < 0.05, **P < 0.01.
Jc 1 Mitochondrial Membrane Potential Assay Kit, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolcarbocyanine iodide (jc-1)  (Cayman Chemical)
90
Cayman Chemical 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolcarbocyanine iodide (jc-1)
Fig. 2. Silica exposure induces <t>mitochondrial</t> depolarization and disrupts energy homeostasis in RAW-ASC cells. RAW-ASC cells were primed with LPS for 6 h then treated with nano-silica, micro-silica, and ATP for 4 h, respectively. (A) Representative images of JC-1 staining showing the alteration of mitochondrial membrane potential between groups. Red, JC-1 aggregates; Green, JC-1 monomers. Scale bar = 50 µm. (B) Quantitative analysis and comparison of fluorescence intensity in A between groups (n = 6). (C) Measurement and comparison of intracellular ROS content between groups (n = 6). (D) Measurement and comparison of intracellular ATP content between groups (n = 4). ns, not significant, *P < 0.05, **P < 0.01.
5,5′,6,6′ Tetrachloro 1,1′,3,3′ Tetraethylbenzimidazolcarbocyanine Iodide (Jc 1), supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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jc-1 mitochondrial membrane potential assay kit  (KeyGene Inc)
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KeyGene Inc jc-1 mitochondrial membrane potential assay kit
Fig. 2. Silica exposure induces <t>mitochondrial</t> depolarization and disrupts energy homeostasis in RAW-ASC cells. RAW-ASC cells were primed with LPS for 6 h then treated with nano-silica, micro-silica, and ATP for 4 h, respectively. (A) Representative images of JC-1 staining showing the alteration of mitochondrial membrane potential between groups. Red, JC-1 aggregates; Green, JC-1 monomers. Scale bar = 50 µm. (B) Quantitative analysis and comparison of fluorescence intensity in A between groups (n = 6). (C) Measurement and comparison of intracellular ROS content between groups (n = 6). (D) Measurement and comparison of intracellular ATP content between groups (n = 4). ns, not significant, *P < 0.05, **P < 0.01.
Jc 1 Mitochondrial Membrane Potential Assay Kit, supplied by KeyGene Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mitochondrial membrane potential staining kit jc1 kit  (Servicebio Inc)
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Servicebio Inc mitochondrial membrane potential staining kit jc1 kit
Fig. 2. Silica exposure induces <t>mitochondrial</t> depolarization and disrupts energy homeostasis in RAW-ASC cells. RAW-ASC cells were primed with LPS for 6 h then treated with nano-silica, micro-silica, and ATP for 4 h, respectively. (A) Representative images of JC-1 staining showing the alteration of mitochondrial membrane potential between groups. Red, JC-1 aggregates; Green, JC-1 monomers. Scale bar = 50 µm. (B) Quantitative analysis and comparison of fluorescence intensity in A between groups (n = 6). (C) Measurement and comparison of intracellular ROS content between groups (n = 6). (D) Measurement and comparison of intracellular ATP content between groups (n = 4). ns, not significant, *P < 0.05, **P < 0.01.
Mitochondrial Membrane Potential Staining Kit Jc1 Kit, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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jc-1 mitochondrial membrane potential detection kit  (Jiancheng Inc)
90
Jiancheng Inc jc-1 mitochondrial membrane potential detection kit
Fig. 2. Silica exposure induces <t>mitochondrial</t> depolarization and disrupts energy homeostasis in RAW-ASC cells. RAW-ASC cells were primed with LPS for 6 h then treated with nano-silica, micro-silica, and ATP for 4 h, respectively. (A) Representative images of JC-1 staining showing the alteration of mitochondrial membrane potential between groups. Red, JC-1 aggregates; Green, JC-1 monomers. Scale bar = 50 µm. (B) Quantitative analysis and comparison of fluorescence intensity in A between groups (n = 6). (C) Measurement and comparison of intracellular ROS content between groups (n = 6). (D) Measurement and comparison of intracellular ATP content between groups (n = 4). ns, not significant, *P < 0.05, **P < 0.01.
Jc 1 Mitochondrial Membrane Potential Detection Kit, supplied by Jiancheng Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mitochondrial membrane potential assay kit jc-1  (Amyjet Scientific Inc)
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Amyjet Scientific Inc mitochondrial membrane potential assay kit jc-1
Fig. 2. Silica exposure induces <t>mitochondrial</t> depolarization and disrupts energy homeostasis in RAW-ASC cells. RAW-ASC cells were primed with LPS for 6 h then treated with nano-silica, micro-silica, and ATP for 4 h, respectively. (A) Representative images of JC-1 staining showing the alteration of mitochondrial membrane potential between groups. Red, JC-1 aggregates; Green, JC-1 monomers. Scale bar = 50 µm. (B) Quantitative analysis and comparison of fluorescence intensity in A between groups (n = 6). (C) Measurement and comparison of intracellular ROS content between groups (n = 6). (D) Measurement and comparison of intracellular ATP content between groups (n = 4). ns, not significant, *P < 0.05, **P < 0.01.
Mitochondrial Membrane Potential Assay Kit Jc 1, supplied by Amyjet Scientific Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 2. Silica exposure induces mitochondrial depolarization and disrupts energy homeostasis in RAW-ASC cells. RAW-ASC cells were primed with LPS for 6 h then treated with nano-silica, micro-silica, and ATP for 4 h, respectively. (A) Representative images of JC-1 staining showing the alteration of mitochondrial membrane potential between groups. Red, JC-1 aggregates; Green, JC-1 monomers. Scale bar = 50 µm. (B) Quantitative analysis and comparison of fluorescence intensity in A between groups (n = 6). (C) Measurement and comparison of intracellular ROS content between groups (n = 6). (D) Measurement and comparison of intracellular ATP content between groups (n = 4). ns, not significant, *P < 0.05, **P < 0.01.

Journal: Ecotoxicology and environmental safety

Article Title: Mechanistic insights into severe pulmonary inflammation caused by silica stimulation: The role of macrophage pyroptosis.

doi: 10.1016/j.ecoenv.2023.114975

Figure Lengend Snippet: Fig. 2. Silica exposure induces mitochondrial depolarization and disrupts energy homeostasis in RAW-ASC cells. RAW-ASC cells were primed with LPS for 6 h then treated with nano-silica, micro-silica, and ATP for 4 h, respectively. (A) Representative images of JC-1 staining showing the alteration of mitochondrial membrane potential between groups. Red, JC-1 aggregates; Green, JC-1 monomers. Scale bar = 50 µm. (B) Quantitative analysis and comparison of fluorescence intensity in A between groups (n = 6). (C) Measurement and comparison of intracellular ROS content between groups (n = 6). (D) Measurement and comparison of intracellular ATP content between groups (n = 4). ns, not significant, *P < 0.05, **P < 0.01.

Article Snippet: The mouse macrophage cell line RAW264.7 cells stably expressing ASC (RAW-ASC) were obtained from Invivogen (San Diego, CA, USA); Dulbecco’s modified Eagle’s medium of high glucose (DMEM), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody, and goat antirabbit IgG-HRP conjugated secondary antibody were obtained from Servicebio (Wuhan, China); Silica nanoparticles, penicillinstreptomycin, mitochondrial Membrane Potential Assay Kit with JC-1, and adenosine triphosphate (ATP) were purchased from Solarbio (Beijing, China); Brilliant blue G (BBG), lipopolysaccharides (LPS), reactive oxygen assay kit, calcium content chromogenic assay kit, ATP assay kit, and Calcein/PI cell assay kit were purchased from Beyotime (Shanghai, China); KCl was obtained from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China); Fetal bovine serum (FBS) was obtained from BI (Israel); Silica particles of micro-size, and fast green FCF were obtained from Sigma-Aldrich (St. Louis, MO, USA); Primary antibodies of NLRP3, P2X7, Pannexin-1, and IL-1β were purchased from ABclonal (Wuhan, China).

Techniques: Staining, Membrane, Comparison, Fluorescence